Method for purifying β-nicotinamide mononucleotide

How to purify β-nicotinamide mononucleotide NMN Powder? 

Baooute Recommends the 3 steps for your reference :
A: You can use the microfiltration equipment to do the microfiltration and nanofiltration process first and collect the concentrated crude solution.


B. Adjust the pH value of the obtained crude solution to 3-7, and inject the sample on a r eversed-phase high performance liquid chromatography preparation column. The stationary phase is octadecylsilane bonded silica gel, and the mobile phase A is pH3- prepared by hydrochloric acid solution. 7 solution, mobile phase B is ethanol, perform gradient elution purification to obtain a purified sample solution;

C: After nanofiltration process of the purified sample solution with  membrane concentration device,you can lyophilized it with a vacuum freeze dryer and then you can get the  purified β-nicotinamide mononucleotide Raw Powder